Insertion junction: LMJ.RY0402.118822_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118822
Chromosome:chromosome 1
Location:7954589
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g066917 LHCBM1 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACGTGCATCCGAATCTCACCCCACGTTC
Internal bar code:CGCAAATGCGAAGTTGATGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:577
LEAP-Seq percent confirming:99.9551
LEAP-Seq n confirming:4457
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTGTGTTGTCTGGTTG
Suggested primer 2:ACTACCTGGTGGCCTGTGTC