Insertion junction: LMJ.RY0402.118877_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118877
Chromosome:chromosome 17
Location:5677330
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g738600 RLS5 RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGTTCCATGACGTCCCATCCAGAGCCA
Internal bar code:AGCCACATACAGAAGAGCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:787
LEAP-Seq percent confirming:56.5697
LEAP-Seq n confirming:1128
LEAP-Seq n nonconfirming:866
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGCACAAAGTAGGGCAC
Suggested primer 2:GGTACCGCTCCATCAAGGTA