| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.118882 |
| Chromosome: | chromosome 2 |
| Location: | 2849344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCGGTGAGATCTATCGCTATGTAAAATA |
| Internal bar code: | GCTAATCATTATGAGCCGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 85.1429 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGTGCCAAGTTTTGTT |
| Suggested primer 2: | GCAAGGTGGCTCAAAAGAAG |