| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118882 |
| Chromosome: | chromosome 9 |
| Location: | 1746554 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396586 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGGGGCGGTTGGGGGAGAATGGTTCC |
| Internal bar code: | TCTTACGGTGGCGCACTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 11.5243 |
| LEAP-Seq n confirming: | 691 |
| LEAP-Seq n nonconfirming: | 5305 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCGCTTGCAATTATACC |
| Suggested primer 2: | CCCAAGCTTGAAAAGCTCAC |