| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118892 |
| Chromosome: | chromosome 6 |
| Location: | 5229576 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAAGGGTCGACAGTGTACACCTGTGTG |
| Internal bar code: | AATCGCGCTATGGACACGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 56.8158 |
| LEAP-Seq n confirming: | 546 |
| LEAP-Seq n nonconfirming: | 415 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGCTGCTCCATCCTACT |
| Suggested primer 2: | CGTGAGACTGCTTTGGTGAA |