| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.118953 |
| Chromosome: | chromosome_2 |
| Location: | 5348815 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g107050 | DHC5 | Dynein heavy chain 5, inner arm dynein b (monomeric) | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGTCGTCTGTGCCGCCCAGGATGAAGGCG |
| Internal bar code: | GCGGCACTGGATTCTTGCTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGCGCTCAAGGTAAGTC |
| Suggested primer 2: | ACTTCTTCAGATGCGGCTGT |