Insertion junction: LMJ.RY0402.118957_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.118957
Chromosome:chromosome_13
Location:3741374
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g589450 sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GGCGCAGCTGCCCGCGTGCTTTTTCGCAAA
Internal bar code:GTAGTTACCGTCCCCAGCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:385
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGCCTGGTTGTAAGCC
Suggested primer 2:GAGGTAGCTGAACTCCACCG