Insertion junction: LMJ.RY0402.118968_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.118968
Chromosome:chromosome 12
Location:2239543
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g508050 (1 of 1) PF01388//PF12047//PF15612 - ARID/BRIGHT DNA binding domain (ARID) // Cytosine specific DNA methyltransferase replication foci domain (DNMT1-RFD) // WSTF, HB1, Itc1p, MBD9 motif 1 (WHIM1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCAGTAAGCATGCTTCATGTCGCGGAG
Internal bar code:CACGAGAGAGGTACATGTACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:294
LEAP-Seq percent confirming:98.4627
LEAP-Seq n confirming:6469
LEAP-Seq n nonconfirming:101
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCAATCGTGTCCGTTGTG
Suggested primer 2:GCAGAACAGCATGCCACTAA