| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118976 |
| Chromosome: | chromosome 11 |
| Location: | 1437967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467729 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGTGTCCAGCTTGGCGTTCAGGAACT |
| Internal bar code: | GGAACGGAGCGTAATCTTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 98.9345 |
| LEAP-Seq n confirming: | 650 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACACACTGCTACGCCAG |
| Suggested primer 2: | CAGTTGAAGGTGGCGGTATT |