| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118988 |
| Chromosome: | chromosome 6 |
| Location: | 4287625 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278253 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGCCGCTTTCAACTGCTGTGGCCGC |
| Internal bar code: | TCAAGGTATTCCCTGCGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 98.8686 |
| LEAP-Seq n confirming: | 9001 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACGACACGGACGAAGAC |
| Suggested primer 2: | CGTACAACCACAACCACCAG |