Insertion junction: LMJ.RY0402.119007_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119007
Chromosome:chromosome 10
Location:2310371
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g434700 (1 of 11) IPR017956 - AT hook, DNA-binding motif intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTGTGAACGAGTGGCGGCTGACAGCAC
Internal bar code:AAAGTAACTTATGCCGACGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:66
LEAP-Seq percent confirming:85.9944
LEAP-Seq n confirming:307
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCTCTGGATGACGACCT
Suggested primer 2:AGTACTGATGCCGCCAATTC