| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119036 |
| Chromosome: | chromosome 4 |
| Location: | 3801991 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229650 | ADH10 | Alcohol dehydrogenase; (1 of 1) 1.3.1.103 - 2-haloacrylate reductase | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCAGCAATCCGAATGACCACCTTGCCC |
| Internal bar code: | GATAGGGTTGCACGAACGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTCATGCACTACCGTAT |
| Suggested primer 2: | GAAATTGAAGGTGGCAAGGA |