| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119045 |
| Chromosome: | chromosome 16 |
| Location: | 7727122 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691552 | NDA1 | Type-II NADH dehydrogenase; (1 of 3) K17871 - NADH:ubiquinone reductase (non-electrogenic) (ndh1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATACCCCTGTCCGTTTTCGCCCCTGGA |
| Internal bar code: | TCGGTGGGGTCGGTCCCCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 454 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGAGACCTCTGGTTTGC |
| Suggested primer 2: | GCAACGTGCAGGGTAGGTAT |