| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119060 |
| Chromosome: | chromosome 14 |
| Location: | 2546301 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625550 | FAP410 | Flagellar Associated Protein 410; (1 of 60) IPR001611 - Leucine-rich repeat | 3'UTR|outside_mRNA |
| Cre14.g625600 | FHL10 | (1 of 2) PF02010 - REJ domain (REJ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATTACTATCCGGGTTCGCCCAGCTCGC |
| Internal bar code: | GAGCCGTAACACTCCGCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 74.5878 |
| LEAP-Seq n confirming: | 1538 |
| LEAP-Seq n nonconfirming: | 524 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTGTGTGTCTTTCGG |
| Suggested primer 2: | ACCACCCCAACTACAAGCAG |