| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119061 |
| Chromosome: | chromosome_10 |
| Location: | 3991911 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g448850 | FAP135,ODA5AK,CAL5 | Calpain cystein protease and Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCCTCAGTTCTGAAGGCTGCGCAGCTGG |
| Internal bar code: | GCCATCTGAGTGTACCTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 91.3369 |
| LEAP-Seq n confirming: | 1708 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGGCTACGATGACTGC |
| Suggested primer 2: | CCCTGCTCCTTTCTTCCTTT |