Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.119061 |
Chromosome: | chromosome_10 |
Location: | 3991914 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g448850 | FAP135,ODA5AK,CAL5 | Calpain cystein protease and Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGAGATGTGGAAGGCACGAGTTGCGGGTG |
Internal bar code: | GCTGAGCGTGTGTGTATCTCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 703 |
LEAP-Seq percent confirming: | 99.7367 |
LEAP-Seq n confirming: | 7954 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAGTGGCTACGATGACTGC |
Suggested primer 2: | CCCTGCTCCTTTCTTCCTTT |