| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119073 |
| Chromosome: | chromosome 14 |
| Location: | 2986383 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627950 | SRD2 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein; (1 of 1) 1.3.1.22 - 3-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) / Cholestenone 5-alpha-reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAGATGTCAAAAGCCTCCCAGGATGCC |
| Internal bar code: | GAATGTCGACGTGTAGCTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.0033 |
| LEAP-Seq n confirming: | 298 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTGCTGCACTACCTCT |
| Suggested primer 2: | GAACAAAACGACACACACCG |