| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119074 |
| Chromosome: | chromosome 12 |
| Location: | 3540974 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513350 | (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGCGAGCTGCCTCTCATACTTGTTATC |
| Internal bar code: | GTGCGGCTAGGGTTACGTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.875 |
| LEAP-Seq n confirming: | 799 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGGAGCCTCACTTAGGA |
| Suggested primer 2: | CAGTAACTCTTCGGGCAAGC |