Insertion junction: LMJ.RY0402.119087_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119087
Chromosome:chromosome 14
Location:2746842
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g627050 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGAGCCGTATCAGCATGGATCCCAAATT
Internal bar code:TTCCATCAACGCGGCATGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:99.3985
LEAP-Seq n confirming:1983
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACTCCCTCTCACCC
Suggested primer 2:CAATTGACTTTGCCTGCTGA