| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119107 |
| Chromosome: | chromosome_14 |
| Location: | 777115 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612950 | CYG17 | Adenylate/guanylate cyclase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTCTCACTCTGCGACATTATGCCAATTTA |
| Internal bar code: | GGTGGCCGCCGTGCCTTTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGATGTCCAGCAGCGTGTC |
| Suggested primer 2: | GGGCTTCTAGTGCTTGTTCG |