| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119109 |
| Chromosome: | chromosome 6 |
| Location: | 5279963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282700 | (1 of 1) PTHR24414//PTHR24414:SF29 - FAMILY NOT NAMED // SEGMENTATION PROTEIN CAP'N'COLLAR | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGAAAGAGGGCGGCACGCAAGGTTGT |
| Internal bar code: | GCTGATCTGCGGGGCCACGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 98.3974 |
| LEAP-Seq n confirming: | 307 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGAGGCGCAGGAGATG |
| Suggested primer 2: | CCAGACAGGGTCCAGACAGT |