| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119109 |
| Chromosome: | chromosome_11 |
| Location: | 3763182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g483100 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTTCGTGCACGTGTCCACAAAACAGTAC |
| Internal bar code: | GGGAAACGGTTCGCGGTGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 95.6835 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCTGCTGTTGATTGCTG |
| Suggested primer 2: | GCGAATATGGACATCACACG |