| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119112 |
| Chromosome: | chromosome 6 |
| Location: | 3995698 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278198 | (1 of 1) 1.14.11.33//4.2.99.18 - DNA oxidative demethylase / Alkylated DNA repair protein // DNA-(apurinic or apyrimidinic site) lyase / Phage-T4 UV endonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGTCGTCGTTACCGATGTGCGCGGAGT |
| Internal bar code: | AGTGGGCAAGGTCATTGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 99.4904 |
| LEAP-Seq n confirming: | 7029 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGCTATAAGAAGTGCG |
| Suggested primer 2: | AACCGGCTTTTGACTGTGTC |