| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119122 |
| Chromosome: | chromosome 3 |
| Location: | 5452321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185300 | GAK1,GALK1 | (1 of 1) 2.7.1.46 - L-arabinokinase; Galactokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCACCTTCCCCGTTCTCTTGAATGAAC |
| Internal bar code: | TGGGCGGACTAAAAGCGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 96.8785 |
| LEAP-Seq n confirming: | 2576 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATGTCACGGTCGTACTG |
| Suggested primer 2: | GCGTTTACAGGCTCGATAGC |