| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119134 |
| Chromosome: | chromosome 7 |
| Location: | 3774665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338250 | (1 of 4) PF04073 - Aminoacyl-tRNA editing domain (tRNA_edit) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTCGCTACCGTATGGCTGCTACCTCAC |
| Internal bar code: | CCCTCCCAGTCAGATGATGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAACGAGCTAGGGAGGG |
| Suggested primer 2: | TGCCCAGTATTTTACCCTGC |