| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119150 |
| Chromosome: | chromosome 16 |
| Location: | 1253381 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGACCTCTGCTGCGGCTGCACGGCGGTG |
| Internal bar code: | CGCCATCCCATCCCCCGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.9221 |
| LEAP-Seq n confirming: | 3846 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTGGCTCTTAGTGAAG |
| Suggested primer 2: | TGCCTTCTCTCTTGCCGTAT |