| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119157 |
| Chromosome: | chromosome 3 |
| Location: | 5093567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181400 | ADC1 | Aminodeoxychorismate synthase; (1 of 1) 2.6.1.85 - Aminodeoxychorismate synthase / ADC synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGGAGCAGGATGAGCGGGGCGGAGAGG |
| Internal bar code: | GTGGTCCCCACTCTCGCCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 99.7156 |
| LEAP-Seq n confirming: | 1052 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATTGCCAAATGTGTACG |
| Suggested primer 2: | GTTTCTGTGCGTGTGCAACT |