Insertion junction: LMJ.RY0402.119165_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.119165
Chromosome:chromosome 7
Location:5947544
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g354400 CYP743B1,CYP21,CYP743B2,CYP743B3 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAAAACCATGCACTGACGCCCTACCCAA
Internal bar code:TTGACCAAAATCGTTCTAATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:178
LEAP-Seq percent confirming:2.82407
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:2099
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGACCCAGCGCTTATTT
Suggested primer 2:CCCATTACACCACCCATCTC