| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119234 |
| Chromosome: | chromosome_4 |
| Location: | 2602350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g222650 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTTCTGTCCCATCACCATCAGTCCACCA |
| Internal bar code: | CGGTACTGGAACGCGGATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 99.6342 |
| LEAP-Seq n confirming: | 2724 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCGGTTTTGTTACATT |
| Suggested primer 2: | CATTGCGGATTTCTCCTCAT |