Insertion junction: LMJ.RY0402.119236_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119236
Chromosome:chromosome 17
Location:3523087
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g725200 (1 of 2) K05658 - ATP-binding cassette, subfamily B (MDR/TAP), member 1 (ABCB1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGTGCCAGGTTCGACACGGTCTTCATA
Internal bar code:CGCGTCAACAATGCGGACGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:935
LEAP-Seq percent confirming:99.4651
LEAP-Seq n confirming:3347
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCACGACGTCACAGTA
Suggested primer 2:CACCGTGCTTATGGTGATTG