| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119259 |
| Chromosome: | chromosome 6 |
| Location: | 8488614 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307700 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACAGCACCTCTGCCCTACGACTGGAG |
| Internal bar code: | TCCGTTGGCTCCCTTGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 21.5004 |
| LEAP-Seq n confirming: | 1026 |
| LEAP-Seq n nonconfirming: | 3746 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCCTGGAGGTGATGGTG |
| Suggested primer 2: | ACGACCGCATCTTGTTATCC |