Insertion junction: LMJ.RY0402.119269_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119269
Chromosome:chromosome 4
Location:3924742
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g230928 TRL13 (1 of 1) K10758 - thiol oxidase (QSOX); Thioredoxin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGCGCACCTACGCCGTGTCGCCTGTGC
Internal bar code:TAAACGGTGGGTAGCGAAAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:706
LEAP-Seq percent confirming:91.2587
LEAP-Seq n confirming:8874
LEAP-Seq n nonconfirming:850
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTACTGGTACACCGCCT
Suggested primer 2:CCATTGTAACATCGCCCTCT