| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119276 |
| Chromosome: | chromosome 8 |
| Location: | 1145884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362700 | SMM30 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGACCAGCATCAGCACGCCGCAGGGCTT |
| Internal bar code: | GCAATACATTCGGTGGTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1102 |
| LEAP-Seq percent confirming: | 99.6466 |
| LEAP-Seq n confirming: | 19454 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCAAAGTGGCCATCAAG |
| Suggested primer 2: | TTAGACGGTGTCAAGTTGCG |