Insertion junction: LMJ.RY0402.119286_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119286
Chromosome:chromosome 7
Location:492207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g315850 (1 of 2) IPR000104//IPR000945//IPR020683 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTCCGCTACGTTGTTGTCATACAGGCG
Internal bar code:CTTCACGCGGCCATTGGGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:501
LEAP-Seq percent confirming:94.7961
LEAP-Seq n confirming:674
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCTCTGCTACAGTCGCTA
Suggested primer 2:TTATTGCGAGTGCTTGTTCG