Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.119291 |
Chromosome: | chromosome_10 |
Location: | 2919356 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g440250 | PGM19 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCATGCGCCGCCCCCATCCTCCTGCACAC |
Internal bar code: | CTCGGCATTCGTGGCAGCAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 430 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 8 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAGCTTGGCAAAATGACTG |
Suggested primer 2: | CGAAGAACTGAGGCAAGTCC |