| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119292 |
| Chromosome: | chromosome_1 |
| Location: | 1758224 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009400 | NSS2 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAGTGACGCTTATGACTCCCCCGGTGATG |
| Internal bar code: | ACGGCACCAGAGGTACCCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 90.383 |
| LEAP-Seq n confirming: | 4389 |
| LEAP-Seq n nonconfirming: | 467 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGACTAAGCATTCAGCA |
| Suggested primer 2: | TCTGTTCTTCATCCCCAAGG |