| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119294 |
| Chromosome: | chromosome 3 |
| Location: | 8696216 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204241 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGGTCGCCCTCTGCCCCCCGATTGCCC |
| Internal bar code: | AAATAGGAGGAAGGGGGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 98.9241 |
| LEAP-Seq n confirming: | 35122 |
| LEAP-Seq n nonconfirming: | 382 |
| LEAP-Seq n unique pos: | 118 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCCGGATTATAAAAGTG |
| Suggested primer 2: | GTACGGGTACGGGTAGGGTT |