Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.119353 |
Chromosome: | chromosome 2 |
Location: | 6601687 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g117250 | (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAACTCCCCCTCCGCCCCGCACAGGCCA |
Internal bar code: | CTGCGACGTCGTATCAGGGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 331 |
LEAP-Seq percent confirming: | 94.9153 |
LEAP-Seq n confirming: | 896 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACACACACACACACACGC |
Suggested primer 2: | GTTAAGCGGGTGGTTTTGAA |