Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.119354 |
Chromosome: | chromosome_6 |
Location: | 7927936 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g303400 | FAP16 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTGTACTTATGCTGGGCTTCAGCCGCCTC |
Internal bar code: | GGTAAGTAAACCTCGCGAGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 613 |
LEAP-Seq percent confirming: | 98.5075 |
LEAP-Seq n confirming: | 132 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGCTTAGTGCTTATCCAGC |
Suggested primer 2: | GTACGACCTGGAGGACTGGA |