| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119366 |
| Chromosome: | chromosome 14 |
| Location: | 461715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610950 | PBD1 | 20S proteasome beta subunit D, type 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGCTAATTATGGTTGGCATGTGTCTGT |
| Internal bar code: | ACAGAGGTCACCCGGGAGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 99.7314 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTAAGCCAAACCAAGC |
| Suggested primer 2: | CCATCTGGGCCATGAGTAGT |