| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119379 |
| Chromosome: | chromosome 1 |
| Location: | 3198727 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020050 | (1 of 4) IPR000533 - Tropomyosin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGAGTCACCTGTCACACAGGTGTTGG |
| Internal bar code: | GCTAAGTACTGGACTCGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 99.4749 |
| LEAP-Seq n confirming: | 1326 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTAGCCTGATTGAACGC |
| Suggested primer 2: | GCTCTGCAAGTACGCAACAA |