| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119405 |
| Chromosome: | chromosome 12 |
| Location: | 4630920 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522900 | LYR4 | Complex 1 protein (LYR family); (1 of 10) PF05347 - Complex 1 protein (LYR family) (Complex1_LYR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGGTTGGCACGGACCTCCGTCGGCAT |
| Internal bar code: | GAGGGTTTCCACACCCAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 99.9107 |
| LEAP-Seq n confirming: | 2237 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACAACCGCAAAGCAGAG |
| Suggested primer 2: | CAGCTCGCCCTTTAAGAATG |