Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.119405 |
Chromosome: | chromosome_12 |
Location: | 7086998 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g559400 | PDE12 | 3\',5\'-cyclic-nucleotide phosphodiesterase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGCCGCACCCGTGGCCCCGGTGCCTTTGG |
Internal bar code: | CCGGAGCTCCAGGCGGACCGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 147 |
LEAP-Seq percent confirming: | 99.8736 |
LEAP-Seq n confirming: | 1580 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAATTGTCCCGTGTTACGCT |
Suggested primer 2: | AAGAATCCCACCTGGCTCTT |