Insertion junction: LMJ.RY0402.119425_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119425
Chromosome:chromosome 10
Location:3002062
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g440950 FAP192 (1 of 1) PTHR14465//PTHR14465:SF0 - IQ DOMAIN-CONTAINING PROTEIN H // IQ DOMAIN-CONTAINING PROTEIN H; Flagellar Associated Protein 192 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCGGCGACGTCACCACGCGACCCGAGCC
Internal bar code:TTGTCAAACCCGGAGGGAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:789
LEAP-Seq percent confirming:99.5465
LEAP-Seq n confirming:439
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAGAGGGTTCCGCAGTAG
Suggested primer 2:GCGTGACGAGACACAAAGAA