| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119446 |
| Chromosome: | chromosome 16 |
| Location: | 2180503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658250 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGGCGGTGCCCTCGTGTTCGCCGTG |
| Internal bar code: | CCCTTTGGGCTGTCGGGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.0898 |
| LEAP-Seq n confirming: | 2395 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGGTTCAGGAGGGATTG |
| Suggested primer 2: | GAGTCTGGCTCACGACATCA |