| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119477 |
| Chromosome: | chromosome 17 |
| Location: | 2093536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712200 | (1 of 1) K11850 - ubiquitin carboxyl-terminal hydrolase 26/29/37 [EC:3.4.19.12] (USP26_29_37) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATGGGACCCCCCCCCATTCCCATTAC |
| Internal bar code: | CCGGCGGTGGTGTGGCACTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 99.659 |
| LEAP-Seq n confirming: | 2630 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGCGTACATCTCCACC |
| Suggested primer 2: | CGGATTTTTGGGTTACCCTT |