| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119487 |
| Chromosome: | chromosome 13 |
| Location: | 527833 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565250 | (1 of 7) PF00582 - Universal stress protein family (Usp) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGACCATCTTTGTTACCCAAAACCGGT |
| Internal bar code: | GGTACGACGTCGGACTGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.1119 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACCGAACCGGAAACTA |
| Suggested primer 2: | AGTGGGCGTATGAAAGTTGG |