| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119499 |
| Chromosome: | chromosome 6 |
| Location: | 859763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254700 | (1 of 1) K18716 - nucleoporin NUP42 (NUP42, RIP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCCGCACAAGGCGGTTGCGGTTCTGT |
| Internal bar code: | TTGTGACAGACGAGAAAGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.7271 |
| LEAP-Seq n confirming: | 2193 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCCTGTCGTAGTGCTG |
| Suggested primer 2: | GAAGAAGCACGTCTTCAGGG |