Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.119503 |
Chromosome: | chromosome 10 |
Location: | 6238253 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g464700 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCCTTAGCAGGTTGGCATTCTTTCTGG |
Internal bar code: | GCGGCGGACAGGCTCAGGACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 661 |
LEAP-Seq percent confirming: | 99.9438 |
LEAP-Seq n confirming: | 5331 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGCTCAACATGCGGACTT |
Suggested primer 2: | AAGTTGTTGCAAGGGTGTCC |