| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119513 |
| Chromosome: | chromosome 17 |
| Location: | 6995219 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746347 | UNG1 | (1 of 1) 3.2.2.27 - Uracil-DNA glycosylase / Uracil-DNA N-glycosylase; Uracil DNA glycosylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCGCGTCCGGCCTCTCGCCCCGACTGT |
| Internal bar code: | GGAAGCGTCTTCGATCAGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.6901 |
| LEAP-Seq n confirming: | 7077 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAGAGCACAAGGAAGGC |
| Suggested primer 2: | GGAGCCTAGGGTTGAGATCC |